In vitro Symbiosis of Reef-Building Coral Cells With Photosynthetic Dinoflagellates

Coral reefs are the biodiversity hot spots of oceans, but they have suffered from increasing environmental stresses caused principally by anthropogenic global warming. The keystone species coral scleractinian corals, which maintain obligatory symbiotic relationships with photosynthetic dinoflagellates. Understanding cellular and molecular mechanisms symbiosis is therefore essential for future preservation reefs. To date, however, almost no in vitro experimental systems been devised to illuminate such mechanisms. this end, our previous study established stable cell culture lines, including IVB5, originating planula larvae coral, Acropora tenuis. Here, we show that soon after mixture dinoflagellate, Breviolum minutum , flattened amorphous cells endodermal properties exhibited elevated locomotor activity using filopodia lamellipodia interacted Several minutes thereafter, began incorporate B. appeared accomplished within 30 min. Nearly a half had incorporated algal 24 h reproducible manner. harbored gradually became round less mobile, sometimes settled vacuole-like structures cytoplasm. This state was maintained at least month. IVB5 line A. tenuis provides an system explore involved coral-dinoflagellate single-cell level, results may be useful